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Processing of the Donor Tissue
Step 1
Receiving the Eye Ball
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Step 2
Slit Lamp Evaluation for Whole Globe
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Step 3
Corneal Excission
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Step 4
Corneal Endothelium Cell Counting through Specular Microscopy
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Step 5
Serology Testing
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Slit Lamp Evaluation:
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Before excising the cornea, The Eye ball is superficially evaluated under the slit lamp for grading.
- Excellent
- Very Good
- Good
- Fair
- Not suitable for surgery
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Corneal Excision:
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Following slit lamp Evaluation the cornea is excised from the Eye Ball and stored in a preservative medium. The preservative medium used is Mc Carey Kaufmann (M.K) medium wherein the cornea can be stored for a period of 4 days.
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Endothelial cell analysis:
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The endothelial cells of the donor cornea can be viewed under a Specular microscopy to analyse its:
- Cell density - ideally it should be > 2000 (PKP)
- Hexagonality of the cells should be more 50%
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Serology: |
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The 5cc of blood collected from the donor is tested for:
- Human Immuno Deficiency virus (AIDS)
- Hepatits B
- Syphilis
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