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ยป Processing
 
     
  Processing of the Donor Tissue
Step 1

Receiving the Eye Ball
Step 2

Slit Lamp Evaluation for Whole Globe
Step 3

Corneal Excission
Step 4

Corneal Endothelium Cell Counting through Specular Microscopy
Step 5

Serology Testing
 
Slit Lamp Evaluation:
Before excising the cornea, The Eye ball is superficially evaluated under the slit lamp for grading.
  • Excellent
  • Very Good
  • Good
  • Fair
  • Not suitable for surgery
Corneal Excision:
Following slit lamp Evaluation the cornea is excised from the Eye Ball and stored in a preservative medium. The preservative medium used is Mc Carey Kaufmann (M.K) medium wherein the cornea can be stored for a period of 4 days.

Endothelial cell analysis:
The endothelial cells of the donor cornea can be viewed under a Specular microscopy to analyse its:
  • Cell density - ideally it should be > 2000 (PKP)
  • Hexagonality of the cells should be more 50%
Serology:
The 5cc of blood collected from the donor is tested for:
  • Human Immuno Deficiency virus (AIDS)
  • Hepatits B
  • Syphilis